Campy Blood-Free Selective Medium (CCDA) is used with cefoperazone for the selective isolation of Campylobacter spp. in a laboratory setting. Campy Blood-Free Selective Medium (CCDA) is not intended for use in the diagnosis of disease or other conditions in humans. Campy Blood-Free Selective Medium (CCDA) was described by Bolton et al. This medium was formulated to replace blood with charcoal, ferrous sulfate, and sodium pyruvate. To improve selectivity, cefoperazone replaced cephazolin in the original formulation. Bolton et al recommended incubating inoculated plates at 37°C to improve isolation rates. Yeast and fungal contaminants are inhibited with the addition of amphotericin B. Campy Blood-Free Selective Medium (CCDA) is recommended for food testing.
- Analyte: campylobacter spp.
- Platform: dehydrated culture media
- Formula: nutrient broth no. 2 25.0g/L, charcoal 4.0g/L, casein acid hydrolysate 3.0g/L, sodium deoxycholate 1.0g/L, ferrous sulfate 0.25g/L, sodium pyruvate 0.25g/L, agar 12.0g/L
- Grams per litre: 45.50
- Litres per 500g: 11.00
- Plates per 500g: 600.00
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